| ID |
U0007 |
|
| Creation Date |
30 September 1997 |
| Updating Date |
30 September 1997 |
|
| Standard Name |
TGE translational regulation element (TGE) |
| UTRSite Pattern |
suca 5...14 mm uuuc yy 4...9 uwucu
|
| Random Expectation |
9.0000000000 hits/kb |
|
| UTR Region |
3' |
| Taxon Range |
Metazoa |
|
| Description |
In the nematode C. elegans sex determining gene tra-2 is
involved in the specification of hermaphrodite rather that
female development.
Negative regulation of tra-2 allow development of XX animals
as hermaphrodite rather than female.
This regulation is in both germ line and soma and it is
necessary for normal male development.
A regulatory element in the tra-2 3'-UTR, consisting
in a 28 nt direct repeat, also termed DRE (Direct Repeat
Element), has been found to be necessary for negative
regulation of tra-2 allowing the onset of spermatogenesis.
Regulation is mediated at translational level, probably
by a trans-acting protein factor called DRF (direct Repeat
Factor) with binding activity to DRE.
DRE consists in two copies of a 28 nt sequence separated by
4 nt. It has been proposed that when no DRF is bound,
translation is not inhibited. Binding of a single repeat
cause partial regulation, and the binding to both repeats
causes full inhibition. No stable secondary structure
was predicted for the DREs by computer analysis.
Elements functionally equivalent to DREs have been found in
the tra-2 gene of the nematode C. Briggsae and in the human
oncogene GLI. For this reason DRE has been renamed TGE
(tra-2 and GLI elements).
Possible mechanisms are that these regulatory elements
may control the length of the poly-A tail, which in turn
may influence translation. Alternatively, the TGEs may
sequester mRNA away from the translation machinery or
they can directly inhibit the interaction of initiation factors
or of ribosomal subunits with the 5'-UTR.
|
|
|
|
| Feature Key |
TGE |
|
| UTR(s) |
| Description |
Species |
Link |
Ref. |
| 3'UTR in Caenorhabditis elegans TRAnsformer XX animals transformed into males family member (tra-2), mRNA |
Caenorhabditis elegans |
UTRef: 3CELR002296 |
[U1] |
|
| Transcript(s) |
| Description |
Species |
Link |
Ref. |
| Caenorhabditis elegans TRAnsformer : XX animals transformed into males family member (tra-2) (tra-2) mRNA, complete cds. |
Caenorhabditis elegans |
RefSeq: NM_063025 |
|
|
| Gene(s) |
|
|
| ID |
[1] |
| Authors |
Goodwin EB, Okkema PG, Evans TC and Kimble J |
| Title |
Translational regulation of tra-2 by its 3' untranslated region controls sexual identity in C. elegans |
| Citation |
Cell 75: 329-339 |
| Year |
1993 |
| ID |
[U1] |
| Authors |
Goodwin EB, Okkema PG, Evans TC and Kimble J |
| Title |
Translational regulation of tra-2 by its 3' untranslated region controls sexual identity in C. elegans |
| Citation |
Cell 75: 329-339 |
| Year |
1993 |
