| ID |
U0034 |
|
| Creation Date |
27 July 2004 |
| Updating Date |
28 September 2009 |
|
| Standard Name |
Gamma interferon activated inhibitor of translation (GAIT element) |
| UTRSite Pattern |
AANGNUUACUUUGGAAUGRCUAUAMNCRUU[1,1,1] |
|
|
| UTR Region |
3' |
| Taxon Range |
Metazoa |
|
| Description |
Delayed translational silencing of ceruloplasmin (Cp) mRNA by gamma-interferon (IFN-gamma) is directed by a structural element in its 3' untranslated region (Mazumder, 1999).
Translational silencing requires the binding of a cytosolic inhibitor to the Cp 3'UTR and all essential elements of mRNA circularization, i.e. eukaryotic initiation factor 4G, poly-A binding protein and poly-A tail (Mazumder, 2001;?
Mazumder, Seshadri, Fox, 2003).
The 29-nt structural element, denoted as IFN-gamma-activated inhibitor of translation (GAIT), consisting of a 5-nt terminal loop, a weak 3-bp helix, an asymmetric internal bulge and a proximal 6-bp helix, has been experimentally demonstrated to be sufficient for translational silencing both in vitro and in vivo (Sampath, 2003). A similar element can be found in sheep Cp but not in rodents. The GAIT element-binding complex consists of at least four proteins: Glutamyl-prolyl RNA synthetase, glyceraldehyde 3-phosphate dehydrogenase, NS1-associated protein 1, and ribosomal protein L13a (Mazumder, Sampath et al, 2003; Sampath, 2004).
|
|
|
|
| Feature Key |
GAIT |
|
| Link |
|
|
| UTR(s) |
| Description |
Species |
Link |
Ref. |
| 3' UTR in Homo sapiens ceruloplasmin (ferroxidase) (CP), mRNA |
|
UTRef: 3HSAR033294 |
|
|
| Transcript(s) |
| Description |
Species |
Link |
Ref. |
| Homo sapiens ceruloplasmin (ferroxidase) (CP), mRNA |
Homo sapiens |
RefSeq: NM_000096 |
|
|
| Gene(s) |
|
|
| Binding Protein(s) |
| Description |
Species |
Link |
Ref. |
| Glutamyl-prolyl tRNA synthetase |
Homo sapiens |
UniProt: P07814 |
[B1] |
|
| Interactor(s) of Binding Protein(s) |
|
|
| ID |
[1] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Fox PL. |
| Title |
Transcript-selective translational silencing by gamma interferon is directed by a novel structural element in the ceruloplasmin mRNA 3' untranslated region. |
| Citation |
Mol. Cell. Biol. 23:1509-19. |
| Year |
2003 |
| ID |
[2] |
| Authors |
Mazumder B, Fox PL |
| Title |
Delayed translational silencing of ceruloplasmin transcript in gamma interferon-activated U937 monocytic cells: Role of the 3' untranslated region |
| Citation |
Mol. Cell. Biol. 19:6898-6905. |
| Year |
1999 |
| ID |
[3] |
| Authors |
Mazumder B, Seshadri V, Imataka H, Sonenberg N, Fox PL |
| Title |
Translational silencing of cerulo-plasmin requires the essential elements of mRNA circularization: Poly(A) tail, poly(A)-binding protein, and eukaryotic translation initiation factor 4G |
| Citation |
Mol. Cell. Biol. 21:6440-9. |
| Year |
2001 |
| ID |
[4] |
| Authors |
Mazumder B, Seshadri V, Fox PL |
| Title |
Translational control by the 3'-UTR: the ends specify the means |
| Citation |
Trends Biochem. Sci. 28:91-8. |
| Year |
2003 |
| ID |
[5] |
| Authors |
Mazumder B, Sampath P, Seshadri V, Maitra RK, DiCorleto PE, Fox PL. |
| Title |
Regulated release of L13a from the 60S ribosomal subunit as a mechanism of transcript-specific translational control |
| Citation |
Cell 115:187-98. |
| Year |
2003 |
| ID |
[6] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL |
| Title |
Noncanonical function of glutamyl-prolyl-tRNA synthetase: Gene-specific silencing of translation |
| Citation |
Cell 119:195-208. |
| Year |
2004 |
| ID |
[B1] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL |
| Title |
Noncanonical function of glutamyl-prolyl-tRNA synthetase: Gene-specific silencing of translation |
| Citation |
Cell 119:195-208. |
| Year |
2004 |
| ID |
[P1] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL |
| Title |
Noncanonical function of glutamyl-prolyl-tRNA synthetase: Gene-specific silencing of translation |
| Citation |
Cell 119:195-208. |
| Year |
2004 |
| ID |
[P2] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL |
| Title |
Noncanonical function of glutamyl-prolyl-tRNA synthetase: Gene-specific silencing of translation |
| Citation |
Cell 119:195-208. |
| Year |
2004 |
| ID |
[P3] |
| Authors |
Mazumder B, Sampath P, Seshadri V, Maitra RK, DiCorleto PE, Fox PL. |
| Title |
Regulated release of L13a from the 60S ribosomal subunit as a mechanism of transcript-specific translational control |
| Citation |
Cell 115:187-98. |
| Year |
2003 |
| ID |
[P4] |
| Authors |
Sampath P, Mazumder B, Seshadri V, Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL |
| Title |
Noncanonical function of glutamyl-prolyl-tRNA synthetase: Gene-specific silencing of translation |
| Citation |
Cell 119:195-208. |
| Year |
2004 |
